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Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.

Analytical high-pressure anion-exchange chromatography on RPC-5 has been used to study the behaviour of a good primer, d(pT-T-A-G), and a poor primer, d(pT-T-T-T-T-T) in the E. coli polynucleotide phosphorylase-catalysed reactions of dADP, dCDP, dGDP and dTDP where the primer is extended, predominan...

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Bibliografiske detaljer
Main Authors: Trip, E M, Smith, M
Format: Artigo
Sprog:Inglês
Udgivet: 1978
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC342101/
https://ncbi.nlm.nih.gov/pubmed/351563
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