Reverse transcription of tRNA.
The 3' terminus of tRNA was enzymatically elongated by an oligo(A) tail. A fragment of DNA polymerase I (E. coli) was used in the presence of manganese to phase and synthesize a cleavable primer at the oligo(A)-tRNA template. When the threedimensional structure of oligo(A)-tRNA is being unfolded und...
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| Udgivet i: | Nucleic Acids Res |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1978
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342068/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/77522/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.4.1165 |
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