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Reverse transcription of tRNA.
The 3' terminus of tRNA was enzymatically elongated by an oligo(A) tail. A fragment of DNA polymerase I (E. coli) was used in the presence of manganese to phase and synthesize a cleavable primer at the oligo(A)-tRNA template. When the threedimensional structure of oligo(A)-tRNA is being unfolde...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1978
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC342068/ https://ncbi.nlm.nih.gov/pubmed/77522 |
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