Establishment of Reverse Direct ELISA and Its Application in Screening High-Affinity Monoclonal Antibodies

Traditionally monoclonal antibody (MAb) titer is determined by indirect enzyme-linked immunosorbent assay (ELISA), which is primarily used to evaluate the quality of MAbs. In this study, the titer and affinity of a group of MAbs against ovalbumin (OVA) were tested by indirect ELISA and the ELISA met...

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Detalhes bibliográficos
Main Authors: Zhang, Chunmei, Li, Rende, Li, Yongming, Song, Chaojun, Liu, Zhijia, Zhang, Yun, Xu, Zhuwei, Zhuang, Ran, Yi, Jing, Yang, Angang, Yang, Kun, Jin, Boquan
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2012
Assuntos:
Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3420550/
https://ncbi.nlm.nih.gov/pubmed/22894783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hyb.2012.0010
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