Polynucleotide kinase from a T4 mutant which lacks the 3' phosphatase activity.
Polynucleotide kinase from E. coli infected with the PseT 1 mutant of bacteriophage T4 has been isolated. The PseT 1 enzyme purifies similarly to normal polynucleotide kinase and effectively transfers the gamma phosphate of ATP to the 5' terminal hydroxyl of DNA and RNA. The PseT 1 and normal enzyme...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1978
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342026/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/205838/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.3.825 |
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