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Polynucleotide kinase from a T4 mutant which lacks the 3' phosphatase activity.

Polynucleotide kinase from E. coli infected with the PseT 1 mutant of bacteriophage T4 has been isolated. The PseT 1 enzyme purifies similarly to normal polynucleotide kinase and effectively transfers the gamma phosphate of ATP to the 5' terminal hydroxyl of DNA and RNA. The PseT 1 and normal enzyme...

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Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Cameron, V, Soltis, D, Uhlenbeck, O C
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1978
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342026/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/205838/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/5.3.825
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