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Centrosome maturation: Measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1

Understanding how cells regulate microtubule nucleation during the cell cycle has been limited by the inability to directly observe nucleation from the centrosome. To view nucleation in living cells, we imaged GFP-tagged EB1, a microtubule tip-binding protein, and determined rates of nucleation by c...

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Autors principals: Piehl, Michelle, Tulu, U. Serdar, Wadsworth, Pat, Cassimeris, Lynne
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC341778/
https://ncbi.nlm.nih.gov/pubmed/14747658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0308205100
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