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Centrosome maturation: Measurement of microtubule nucleation throughout the cell cycle by using GFP-tagged EB1

Understanding how cells regulate microtubule nucleation during the cell cycle has been limited by the inability to directly observe nucleation from the centrosome. To view nucleation in living cells, we imaged GFP-tagged EB1, a microtubule tip-binding protein, and determined rates of nucleation by c...

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Detalhes bibliográficos
Main Authors: Piehl, Michelle, Tulu, U. Serdar, Wadsworth, Pat, Cassimeris, Lynne
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC341778/
https://ncbi.nlm.nih.gov/pubmed/14747658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0308205100
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