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Development of an Efficient In Vivo System (P(junc)-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei
The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei,...
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| Главные авторы: | , , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2012
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3416393/ https://ncbi.nlm.nih.gov/pubmed/22610425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00531-12 |
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