Development of an Efficient In Vivo System (P(junc)-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei

The random transposon mutagenesis system P(junc)-TpaseIS(1223) is composed of plasmids pVI129, expressing IS1223 transposase, and pVI110, a suicide transposon plasmid carrying the P(junc) sequence, the substrate of the IS1223 transposase. This system is particularly efficient in Lactobacillus casei,...

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Detalhes bibliográficos
Main Authors: Licandro-Seraut, Hélène, Brinster, Sophie, van de Guchte, Maarten, Scornec, Hélène, Maguin, Emmanuelle, Sansonetti, Philippe, Cavin, Jean-François, Serror, Pascale
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416393/
https://ncbi.nlm.nih.gov/pubmed/22610425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00531-12
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