Development of a Double-Crossover Markerless Gene Deletion System in Bifidobacterium longum: Functional Analysis of the α-Galactosidase Gene for Raffinose Assimilation

Functional analysis of Bifidobacterium genes is essential for understanding host-Bifidobacterium interactions with beneficial effects on human health; however, the lack of an effective targeted gene inactivation system in bifidobacteria has prevented the development of functional genomics in this ba...

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Detalhes bibliográficos
Main Authors: Hirayama, Yosuke, Sakanaka, Mikiyasu, Fukuma, Hidenori, Murayama, Hiroki, Kano, Yasunobu, Fukiya, Satoru, Yokota, Atsushi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416363/
https://ncbi.nlm.nih.gov/pubmed/22582061
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00588-12
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