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Synthesis of Long-Chain Chitooligosaccharides by a Hypertransglycosylating Processive Endochitinase of Serratia proteamaculans 568

We describe the heterologous expression and characterization of a 407-residue single-domain glycosyl hydrolase family 18 chitinase (SpChiD) from Gram-negative Serratia proteamaculans 568 that has unprecedented catalytic properties. SpChiD was optimally active at pH 6.0 and 40°C, where it showed a K(...

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Bibliografiske detaljer
Main Authors: Purushotham, Pallinti, Podile, Appa Rao
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416260/
https://ncbi.nlm.nih.gov/pubmed/22685288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.06473-11
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