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SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poor...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lindner, Heike, Raissig, Michael T., Sailer, Christian, Shimosato-Asano, Hiroko, Bruggmann, Rémy, Grossniklaus, Ueli
Format: Artigo
Sprache:Inglês
Veröffentlicht: Genetics Society of America 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3416015/
https://ncbi.nlm.nih.gov/pubmed/22649081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.112.141341
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