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Quantitation of lysolipids, fatty acids, and phospholipase A(2) activity and correlation with membrane polarity
Acrylodan-labeled rat-intestinal fatty acid binding protein, ADIFAB, binds both of lysophosphatidylcholines (LPC) and FA. Binding displaces Acrylodan and its fluorescence peak shifts from 432 to 505 nm. A fluorescence assay that relies on this shift is presented for quantitating LPC, FA, and phospho...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Biochemistry and Molecular Biology
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3413239/ https://ncbi.nlm.nih.gov/pubmed/22773689 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D028746 |
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