Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation.
This communication describes an assay for the relative translation efficiency of individual codons which makes use of the pyrE attenuator to probe the coupling between transcription and translation at the end of an artificial leader peptide. By cloning of short synthetic DNA fragments the codons to...
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| Опубликовано в:: | Nucleic Acids Res |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Oxford University Press
1985
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| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341299/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989788/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.11.4113 |
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