Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation.
This communication describes an assay for the relative translation efficiency of individual codons which makes use of the pyrE attenuator to probe the coupling between transcription and translation at the end of an artificial leader peptide. By cloning of short synthetic DNA fragments the codons to...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1985
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341299/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989788/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.11.4113 |
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