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Rapid assay for detection of Escherichia coli xanthine-guanine phosphoribosyltransferase activity in transduced cells.

Cultured mammalian cells transduced with the Escherichia coli gene, Ecogpt, synthesize the bacterial enzyme xanthine-guanine phosphoribosyl transferase (XGPT) (1). This paper describes a method for measuring XGPT activity in crude cell extracts by following the conversion of 14C-xanthine (X) to 14C-...

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書目詳細資料
發表在:Nucleic Acids Res
Main Authors: Chu, G, Berg, P
格式: Artigo
語言:Inglês
出版: Oxford University Press 1985
在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341204/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889850/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.8.2921
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