Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormon...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Buell, G, Schulz, M F, Selzer, G, Chollet, A, Movva, N R, Semon, D, Escanez, S, Kawashima, E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1985
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341125/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.6.1923
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