Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).
Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormon...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , , , , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
1985
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| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341125/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.6.1923 |
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