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A simple and efficient method for chemical mutagenesis of DNA.
A simple and efficient procedure for the generation of random GC to AT transition mutations in a specific DNA segment is described. A restriction fragment is inserted in each orientation into an M13 vector, single-stranded virion DNA from each recombinant phage is treated with methoxylamine, and, af...
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| Главные авторы: | , |
|---|---|
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
1985
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| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC341108/ https://ncbi.nlm.nih.gov/pubmed/3889841 |
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