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A simple and efficient method for chemical mutagenesis of DNA.

A simple and efficient procedure for the generation of random GC to AT transition mutations in a specific DNA segment is described. A restriction fragment is inserted in each orientation into an M13 vector, single-stranded virion DNA from each recombinant phage is treated with methoxylamine, and, af...

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Bibliografske podrobnosti
Main Authors: Kadonaga, J T, Knowles, J R
Format: Artigo
Jezik:Inglês
Izdano: 1985
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC341108/
https://ncbi.nlm.nih.gov/pubmed/3889841
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