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Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy

Fluorophore excited state lifetime is a useful indicator of micro-environment in cellular optical molecular imaging. For quantitative sensing, precise lifetime determination is important, yet is often difficult to accomplish when using the experimental conditions favored by live cells. Here we repor...

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Detalhes bibliográficos
Main Authors: Chang, Ching-Wei, Mycek, Mary-Ann
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3410727/
https://ncbi.nlm.nih.gov/pubmed/20588712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.18.008688
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