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Intracellular single molecule microscopy reveals two kinetically distinct pathways for microRNA assembly
MicroRNAs (miRNAs) associate with components of the RNA-induced silencing complex (RISC) to assemble on mRNA targets and regulate protein expression in higher eukaryotes. Here we describe a method for the intracellular single-molecule, high-resolution localization and counting (iSHiRLoC) of miRNAs....
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3410386/ https://ncbi.nlm.nih.gov/pubmed/22688967 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/embor.2012.85 |
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