Intracellular single molecule microscopy reveals two kinetically distinct pathways for microRNA assembly

MicroRNAs (miRNAs) associate with components of the RNA-induced silencing complex (RISC) to assemble on mRNA targets and regulate protein expression in higher eukaryotes. Here we describe a method for the intracellular single-molecule, high-resolution localization and counting (iSHiRLoC) of miRNAs....

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Bibliografiske detaljer
Main Authors: Pitchiaya, Sethuramasundaram, Androsavich, John R, Walter, Nils G
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2012
Fag:
Scientific Reports
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3410386/
https://ncbi.nlm.nih.gov/pubmed/22688967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/embor.2012.85
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