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Substrate specificity of DNA polymerases. I. Enzyme-catalysed incorporation of 5-'1-alkenyl)-2'-deoxyuridines into DNA.

A series of (E)-5-(1-alkenyl)-dUTPs as well as 5-vinyl-and (Z)-5-(1-propenyl)-dUTP have been synthesized to study steric requirements in DNA polymerase reactions. Experiments were carried out in E. coli DNA polymerase I Klenow fragment enzyme system. Substrates were characterized by KM and Vmax-valu...

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Bibliografske podrobnosti
Main Authors: Otvös, L, Sági, J, Kovács, T, Walker, R T
Format: Artigo
Jezik:Inglês
Izdano: 1987
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC340580/
https://ncbi.nlm.nih.gov/pubmed/3822838
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