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Plasmid mediated mutagenesis of a cellular gene in transfected eukaryotic cells.
NIH3T3 cells are widely used in transformation assays and readily take up transfected DNA. A system has been devised using NIH3T3 cells to measure the mutagenic effect of transfected DNA on recipient cell genes. NIH3T3 cells can be mutated to 6-thioguanine resistance at a frequency which suggests th...
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| Main Authors: | , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1987
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340452/ https://ncbi.nlm.nih.gov/pubmed/3469621 |
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