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Footprinting at low temperatures: evidence that ethidium and other simple intercalators can discriminate between different nucleotide sequences.
Footprinting experiments employing DNAase I have been performed at 4 degrees C. At this temperature several simple intercalating ligands, including both ethidium and proflavine, can be seen to induce marked changes in the pattern of cleavage. From an analysis of the changes in patterns of digestion...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1987
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340448/ https://ncbi.nlm.nih.gov/pubmed/3822813 |
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