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Fast and Non-Toxic In Situ Hybridization without Blocking of Repetitive Sequences
Formamide is the preferred solvent to lower the melting point and annealing temperature of nucleic acid strands in in situ hybridization (ISH). A key benefit of formamide is better preservation of morphology due to a lower incubation temperature. However, in fluorescence in situ hybridization (FISH)...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3404051/ https://ncbi.nlm.nih.gov/pubmed/22911704 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040675 |
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