Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3′-end-seq

Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and...

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Autori principali: Haenni, Simon, Ji, Zhe, Hoque, Mainul, Rust, Nigel, Sharpe, Helen, Eberhard, Ralf, Browne, Cathy, Hengartner, Michael O., Mellor, Jane, Tian, Bin, Furger, André
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2012
Soggetti:
RNA
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3401467/
https://ncbi.nlm.nih.gov/pubmed/22467213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks282
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