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Reevaluation of ANS Binding to Human and Bovine Serum Albumins: Key Role of Equilibrium Microdialysis in Ligand – Receptor Binding Characterization

In this work we return to the problem of the determination of ligand–receptor binding stoichiometry and binding constants. In many cases the ligand is a fluorescent dye which has low fluorescence quantum yield in free state but forms highly fluorescent complex with target receptor. That is why many...

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Hlavní autoři: Kuznetsova, Irina M., Sulatskaya, Anna I., Povarova, Olga I., Turoverov, Konstantin K.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3400656/
https://ncbi.nlm.nih.gov/pubmed/22829890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040845
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