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There exists a distinct stage during mammalian DNA synthesis immediately after joining of replication intermediates.
We describe an approach, using alkaline cell lysis and digestion with nuclease S1, which permits to distinguish between newly ligated DNA and the DNA of mature chromatin. When cells with steady-state labelled DNA (mature DNA) are analyzed, the results show labelled "nucleosomal-sized" DNA....
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1986
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC339822/ https://ncbi.nlm.nih.gov/pubmed/3012484 |
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