Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores

A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored...

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Main Authors: Dougherty, Joseph D., Zhang, Juliet, Feng, Huifen, Gong, Shiaoching, Heintz, Nathaniel
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2012
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3395707/
https://ncbi.nlm.nih.gov/pubmed/22808177
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0040511
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