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Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.

The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc7GTP) in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nucl...

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Detalles Bibliográficos
Main Authors: Mizusawa, S, Nishimura, S, Seela, F
Formato: Artigo
Idioma:Inglês
Publicado: 1986
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC339506/
https://ncbi.nlm.nih.gov/pubmed/3951988
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