Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.
The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc7GTP) in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nucl...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1986
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC339506/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3951988/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.3.1319 |
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