Quantification of polymerase chain reaction products by affinity-based hybrid collection.
We have used oligonucleotides modified with biotin in the 5'-end as primers in the polymerase chain reaction (PCR)-amplification. This results in the synthesis of 5'-biotinylated DNA molecules, which are detected by hybridization to a labelled probe in solution. The formed hybrids are collected on a...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1988
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC339013/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2849762/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.23.11327 |
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