Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiation in situ.
Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existenc...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1988
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338992/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2974540/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.23.10985 |
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