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Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiation in situ.

Protein-RNA interactions in small nuclear ribonucleoproteins (UsnRNPs) from HeLa cells were investigated by irradiation of purified nucleoplasmic snRNPs U1 to U6 with UV light at 254 nm. The cross-linked proteins were analyzed on one- and two-dimensional gel electrophoresis systems, and the existenc...

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Publicat a:Nucleic Acids Res
Autors principals: Woppmann, A, Rinke, J, Lührmann, R
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1988
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338992/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2974540/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.23.10985
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