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Crystallization of SHARPIN using an automated two-dimensional grid screen for optimization
An N-terminal fragment of human SHARPIN was recombinantly expressed in Escherichia coli, purified and crystallized. Crystals suitable for X-ray diffraction were obtained by a one-step optimization of seed dilution and protein concentration using a two-dimensional grid screen. The crystals belonged t...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
International Union of Crystallography
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3388930/ https://ncbi.nlm.nih.gov/pubmed/22750873 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112022208 |
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