Cloning, purification, crystallization and preliminary X-ray diffraction crystallographic study of acyl-protein thioesterase 1 from Saccharomyces cerevisiae

Palmitoylation/depalmitoylation plays an important role in protein modification. yApt1 is the only enzyme in Saccharomyces cerevisiae that catalyses depalmitoylation. In the present study, recombinant full-length yApt1 was cloned, expressed, purified and crystallized. The crystals diffracted to 2.40...

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Autores principales: Yuan, Ye, Wang, Xiao, Li, Xu, Teng, Maikun, Niu, Liwen, Gao, Yongxiang
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2012
Materias:
Crystallization Communications
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388919/
https://ncbi.nlm.nih.gov/pubmed/22750862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112019276
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