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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of 5-­aminolaevulinic acid dehydratase from Bacillus subtilis

5-Aminolaevulinic acid dehydratase (ALAD), a crucial enzyme in the biosynthesis of tetrapyrrole, catalyses the condensation of two 5-aminolaevulinic acid (ALA) molecules to form porphobilinogen (PBG). The gene encoding ALAD was amplified from genomic DNA of Bacillus subtilis and the protein was over...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lu, Qianda, Ma, Jinming, Rong, Hui, Fan, Jun, Yuan, Ye, Li, Kuai, Gao, Yongxiang, Zhang, Xiao, Teng, Maikun, Niu, Liwen
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935225/
https://ncbi.nlm.nih.gov/pubmed/20823524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110027582
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