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Use of PCR Template-Derived Probes Prevents Off-Target Whole Mount In Situ Hybridization in Transgenic Zebrafish

Transgenic zebrafish have been utilized for in vivo analysis of cell behaviors using advanced imaging techniques, for analyzing spatiotemporal gene regulation, and for targeted mis-expression of transgenes. The Tg(fli1a:EGFP)(y1) vascular reporter has been particularly useful for examining the devel...

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Detalhes bibliográficos
Main Authors: Cha, Young R., Weinstein, Brant M.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388497/
https://ncbi.nlm.nih.gov/pubmed/22715949
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/zeb.2011.0731
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