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Construction of an expression vector for the fission yeast Schizosaccharomyces pombe.
We have isolated and characterized a S. pombe promoter using a functional heterologous gene product assay. Random S. pombe genomic fragments were cloned upstream from the promoterless 'lacZ gene and tested in vivo for their efficiency to promote expression of the beta-galactosidase protein in t...
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| Main Authors: | , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1988
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC338579/ https://ncbi.nlm.nih.gov/pubmed/2843820 |
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