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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe

Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through homologous recombination. Despite the advanta...

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Principais autores: Yasutaka Kakui, Tomonari Sunaga, Kunio Arai, James Dodgson, Liang Ji, Attila Csikász-Nagy, Rafael Carazo-Salas, Masamitsu Sato
Format: Artigo
Sprog:Inglês
Udgivet: The Royal Society 2015-01-01
Serier:Open Biology
Fag:
fission yeast
chromosomal integration
plasmid
cloning
fluorescent protein
Online adgang:https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150054
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