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Oligonucleotide site-directed mutagenesis in Xenopus egg extracts.
Addition of M13mp18 single-stranded DNA annealed with an oligonucleotide to a Xenopus egg extract results in a rapid and efficient incorporation of the oligonucleotide in a complete double-stranded supercoiled molecule. Both the efficiency of DNA synthesis and the recovery of complete double-strande...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1988
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC338574/ https://ncbi.nlm.nih.gov/pubmed/3047681 |
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