Oligonucleotide site-directed mutagenesis in Xenopus egg extracts.
Addition of M13mp18 single-stranded DNA annealed with an oligonucleotide to a Xenopus egg extract results in a rapid and efficient incorporation of the oligonucleotide in a complete double-stranded supercoiled molecule. Both the efficiency of DNA synthesis and the recovery of complete double-strande...
保存先:
| 出版年: | Nucleic Acids Res |
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| 主要な著者: | , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Oxford University Press
1988
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| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338574/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3047681/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.17.8525 |
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