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Oligonucleotide site-directed mutagenesis in Xenopus egg extracts.

Addition of M13mp18 single-stranded DNA annealed with an oligonucleotide to a Xenopus egg extract results in a rapid and efficient incorporation of the oligonucleotide in a complete double-stranded supercoiled molecule. Both the efficiency of DNA synthesis and the recovery of complete double-strande...

詳細記述

保存先:
書誌詳細
主要な著者: Almouzni, G, Mousseron-Grall, S, Méchali, M
フォーマット: Artigo
言語:Inglês
出版事項: 1988
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC338574/
https://ncbi.nlm.nih.gov/pubmed/3047681
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