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Bacillus subtilis genome editing using ssDNA with short homology regions
In this study, we developed a simple and efficient Bacillus subtilis genome editing method in which targeted gene(s) could be inactivated by single-stranded PCR product(s) flanked by short homology regions and in-frame deletion could be achieved by incubating the transformants at 42°C. In this proce...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3384351/ https://ncbi.nlm.nih.gov/pubmed/22422839 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks248 |
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