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Kinetic analysis of an E.coli phenylalanine-tRNA synthetase mutant.

A mutation in the pheS gene, encoding phenylalanyl-tRNA synthetase, in E. coli NP37 confers temperature-sensitivity on the organism. A five-fold increase in tRNA(phe) levels complements the mutation. Analysis of the kinetic properties of the mutant enzyme indicates that the KM is 20-fold higher than...

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書誌詳細
出版年:Nucleic Acids Res
主要な著者: Goodman, R, Schwartz, I
フォーマット: Artigo
言語:Inglês
出版事項: Oxford University Press 1988
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338421/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3045758/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.15.7477
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