Kinetic analysis of an E.coli phenylalanine-tRNA synthetase mutant.
A mutation in the pheS gene, encoding phenylalanyl-tRNA synthetase, in E. coli NP37 confers temperature-sensitivity on the organism. A five-fold increase in tRNA(phe) levels complements the mutation. Analysis of the kinetic properties of the mutant enzyme indicates that the KM is 20-fold higher than...
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| Veröffentlicht in: | Nucleic Acids Res |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1988
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338421/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3045758/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.15.7477 |
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