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A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: evidence that native k1 has novel promoters.
The killer toxin of Kluyveromyces lactis consists of three polypeptides encoded by the linear plasmid k1. We re-introduced the entire k1 sequence, cloned on a circular replicating plasmid, into K. lactis strains lacking k1, and found that the resulting transformants did not produce toxin. The barrie...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1988
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| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC338412/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3412888/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/16.15.7333 |
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