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A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: evidence that native k1 has novel promoters.

The killer toxin of Kluyveromyces lactis consists of three polypeptides encoded by the linear plasmid k1. We re-introduced the entire k1 sequence, cloned on a circular replicating plasmid, into K. lactis strains lacking k1, and found that the resulting transformants did not produce toxin. The barrie...

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Autors principals: Romanos, M A, Boyd, A
Format: Artigo
Idioma:Inglês
Publicat: 1988
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC338412/
https://ncbi.nlm.nih.gov/pubmed/3412888
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