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A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: evidence that native k1 has novel promoters.
The killer toxin of Kluyveromyces lactis consists of three polypeptides encoded by the linear plasmid k1. We re-introduced the entire k1 sequence, cloned on a circular replicating plasmid, into K. lactis strains lacking k1, and found that the resulting transformants did not produce toxin. The barrie...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1988
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC338412/ https://ncbi.nlm.nih.gov/pubmed/3412888 |
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