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Structural interconversion of alternating purine-pyrimidine inverted repeats cloned in supercoiled plasmids.
Two self complementary oligonucleotides, T(GC)4AT(GC)4ACATG and C(GC)2(AT)5 (GC)3ATG, were synthesized and cloned into plasmids. Negative supercoiling causes a structural transition in the primary helix of both inserts. The first sequence converts into the left-handed helix, whereas the second seque...
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| Main Authors: | , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1988
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC338342/ https://ncbi.nlm.nih.gov/pubmed/3405754 |
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