Structural interconversion of alternating purine-pyrimidine inverted repeats cloned in supercoiled plasmids.

Two self complementary oligonucleotides, T(GC)4AT(GC)4ACATG and C(GC)2(AT)5 (GC)3ATG, were synthesized and cloned into plasmids. Negative supercoiling causes a structural transition in the primary helix of both inserts. The first sequence converts into the left-handed helix, whereas the second seque...

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Main Authors: Klysik, J, Zacharias, W, Galazka, G, Kwinkowski, M, Uznanski, B, Okruszek, A
Format: Artigo
Jezik:Inglês
Izdano: 1988
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC338342/
https://ncbi.nlm.nih.gov/pubmed/3405754
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